![]() High rates of somatic mosaicism were induced when embryos were injected with Cas9 ribonucleoprotein complexes (RNPs) pre-assembled with guide RNAs (sgRNAs) at high concentrations. cuprina yellow genes ( ChY and LcY) producing lightly pigmented adults. Site-directed mutations were introduced in the C. Here, we report a significant addition to the pool of genome manipulation tools through the establishment of efficient CRISPR/Cas9 protocols for the generation of directed and inheritable modifications in the genome of these flies. As they are serious pests, considerable effort has been made to develop genomic resources and functional tools aiming to improve their management and control. Their larvae infest warm-blooded vertebrates and feed on host’s tissues, resulting in severe industry losses. Cochliomyia hominivorax and Lucilia cuprina are major pests of livestock.
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